{"id":29,"date":"2022-03-03T13:07:38","date_gmt":"2022-03-03T13:07:38","guid":{"rendered":"https:\/\/celllab.thu.edu.tw\/?p=29"},"modified":"2022-03-03T13:14:56","modified_gmt":"2022-03-03T13:14:56","slug":"29","status":"publish","type":"post","link":"https:\/\/celllab.thu.edu.tw\/index.php\/2022\/03\/03\/29\/","title":{"rendered":"Cell Lab"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">\n<style>\n\t#hotspot-7 .hotspots-image-container,\n\t#hotspot-7 .leaflet-container {\n\t\tbackground: #efefef\t}\n\n\t#hotspot-7 .hotspots-placeholder {\n\t\tbackground: #d8b102;\n\t\tborder: 0 #d8b102 solid;\n\t\tcolor: #DFEBE5;\n\t}\n\n\t#hotspot-7 .hotspot-title {\n\t\tcolor: #3f445b;\n\t}\n\n\t\t\t#hotspot-7 .hotspot-default {\n\t\t\tstroke-width: -1;\n\t\t\tfill: #ffffff;\n\t\t\tfill-opacity: 0;\n\t\t\tstroke: #ffffff;\n\t\t\tstroke-opacity: 0;\n\t\t}\n\t\t#hotspot-7 .hotspot-default:hover,\n\t\t#hotspot-7 .hotspot-default:focus-visible,\n\t\t#hotspot-7 .hotspot-default.hotspot-active {\n\t\t\tfill: #3CA2A2;\n\t\t\tfill-opacity: 0.91;\n\t\t\tstroke: #235B6E;\n\t\t\tstroke-opacity: 1.01;\n\t\t}\n\t\t#hotspot-7 .leaflet-tooltip,\n\t#hotspot-7 .leaflet-rrose-content-wrapper {\n\t\tbackground: #d8b102;\n\t\tborder-color: #d8b102;\n\t\tcolor: #DFEBE5;\n\t}\n\n\t#hotspot-7 a.leaflet-rrose-close-button {\n\t\tcolor: #3f445b;\n\t}\n\n\t#hotspot-7 .leaflet-rrose-tip {\n\t\tbackground: #d8b102;\n\t}\n\n\t#hotspot-7 .leaflet-popup-scrolled {\n\t\tborder-bottom-color: #DFEBE5;\n\t\tborder-top-color: #DFEBE5;\n\t}\n\n\t#hotspot-7 .leaflet-tooltip-top:before {\n\t\tborder-top-color: #d8b102;\n\t}\n\n\t#hotspot-7 .leaflet-tooltip-bottom:before {\n\t\tborder-bottom-color: #d8b102;\n\t}\n\t#hotspot-7 .leaflet-tooltip-left:before {\n\t\tborder-left-color: #d8b102;\n\t}\n\t#hotspot-7 .leaflet-tooltip-right:before {\n\t\tborder-right-color: #d8b102;\n\t}\n<\/style>\n\n\t\n\t<div class=\"hotspots-container  layout-left event-click\" id=\"hotspot-7\" data-layout=\"left\" data-trigger=\"click\">\n\t\t<div class=\"hotspots-interaction\">\n\t\t\t\t<div class=\"hotspots-placeholder\" id=\"content-hotspot-7\">\n\t\t<div class=\"hotspot-initial\">\n\t\t\t\t\t\t\t\t\t<h2 class=\"hotspot-title\">\n\t\t\t\tCell Lab\t\t\t<\/h2>\n\t\t\t\t\t\t<div class=\"hotspot-content\">\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t<\/div>\n<div class=\"hotspots-image-container\">\n\t<img\n\t\twidth=\"1478\"\n\t\theight=\"1108\"\n\t\tsrc=\"https:\/\/i0.wp.com\/celllab.thu.edu.tw\/wp-content\/uploads\/2022\/03\/lab-background.jpeg?fit=1478%2C1108&ssl=1\"\n\t\talt=\"Cell Lab\"\n\t\tclass=\"hotspots-image skip-lazy\"\n\t\tusemap=\"#hotspots-image-7\"\n\t\tdata-image-title=\"Cell Lab\"\n\t\tdata-image-description=\"\"\n\t\tdata-event-trigger=\"click\"\n\t\tdata-always-visible=\"false\"\n\t\tdata-id=\"7\"\n\t\tdata-no-lazy=\"1\"\n\t\tdata-lazy-src=\"\"\n\t\tdata-lazy=\"false\"\n\t\tloading=\"eager\"\n\t\tdata-skip-lazy=\"1\"\n\t\t>\n<\/div>\t\t<\/div>\n\t\t<map name=\"hotspots-image-7\" class=\"hotspots-map\">\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"274,182,316,149,537,148,537,345,503,373,508,505,278,500,275,383,272,268,275,214,275,199\"\n\t\t\t\t\thref=\"#hotspot-hotspot-7-0\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Cell culture Transfection passage\"\n\t\t\t\t\talt=\"Cell culture Transfection passage\"\n\t\t\t\t\tdata-action=\"\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"_new\"\n\t\t\t\t\tclass=\"more-info-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"355,585,364,576,362,521,309,521,265,547,261,607,255,618,254,687,420,691,473,655,472,588,416,587,410,579,399,575,392,583,388,588\"\n\t\t\t\t\thref=\"https:\/\/youtu.be\/tYxo72rBQdE\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Cleaning water bath\"\n\t\t\t\t\talt=\"Cleaning water bath\"\n\t\t\t\t\tdata-action=\"url\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"url-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"751,626,738,615,732,619,723,607,711,604,703,595,689,595,679,607,679,618,688,630,695,637,702,640,698,653,717,670,734,676,741,713,735,727,729,783,779,799,876,723,887,722,887,753,911,749,903,691,918,677,918,646,904,635,897,585,909,577,877,539,870,525,861,506,859,493,832,485,824,487,817,505,816,533,811,546,810,565,817,567,817,597,834,605,848,601,851,614,836,614,817,607,802,608,802,533,694,501,688,505,687,581,755,602\"\n\t\t\t\t\thref=\"https:\/\/www.youtube.com\/watch?v=B364jI3Ei6E&list=PL5r1w-Pm599ezjofhI2yFk4e6lZskfTsL&index=2\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Phase contrast Cell counting Live Image\"\n\t\t\t\t\talt=\"Phase contrast Cell counting Live Image\"\n\t\t\t\t\tdata-action=\"url\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"_new\"\n\t\t\t\t\tclass=\"url-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"935,199,900,232,899,305,899,350,938,354,957,339,959,352,1063,354,1079,350,1100,334,1102,292,1087,284,1043,284,1089,278,1090,198\"\n\t\t\t\t\thref=\"https:\/\/youtu.be\/UuQbWF-oKu0\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"MTT test\"\n\t\t\t\t\talt=\"MTT test\"\n\t\t\t\t\tdata-action=\"url\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"url-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"903,813,902,778,923,751,970,751,983,734,983,728,1008,707,1062,712,1073,718,1068,752,1091,758,1103,740,1144,741,1156,754,1179,762,1194,747,1207,748,1229,750,1241,755,1249,755,1254,764,1252,773,1248,792,1233,805,1186,803,1183,775,1175,769,1146,764,1158,768,1159,779,1165,783,1165,793,1166,800,1151,812,1084,809,1083,792,1090,788,1089,780,1092,773,1104,769,1104,762,1067,754,1059,760,1063,773,1067,778,1068,796,1053,806,987,806,986,788,991,784,992,773,1001,766,991,766,971,757,972,791,948,814\"\n\t\t\t\t\thref=\"#hotspot-hotspot-7-4\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Western blot\"\n\t\t\t\t\talt=\"Western blot\"\n\t\t\t\t\tdata-action=\"\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"more-info-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"555,195,559,189,566,179,607,147,640,148,647,146,643,140,669,146,679,145,715,147,751,147,755,316,707,359,563,361\"\n\t\t\t\t\thref=\"https:\/\/youtu.be\/mT_q-uEa14M\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Wash water plate in incubatot\"\n\t\t\t\t\talt=\"Wash water plate in incubatot\"\n\t\t\t\t\tdata-action=\"url\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"url-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"1193,23,1127,65,1116,68,1116,174,1265,176,1265,68\"\n\t\t\t\t\thref=\"https:\/\/youtu.be\/5mU4cq8PBAA\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Immunofluorescence\"\n\t\t\t\t\talt=\"Immunofluorescence\"\n\t\t\t\t\tdata-action=\"url\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"url-area\"\n\t\t\t\t\t>\n\t\t\t\t\t\t\t\t\t\t\t<area\n\t\t\t\t\tshape=\"polygon\"\n\t\t\t\t\tcoords=\"1118,285,1118,359,1242,361,1266,341,1265,271,1234,267,1155,266\"\n\t\t\t\t\thref=\"#hotspot-hotspot-7-7\"\n\t\t\t\t\trel=\"\"\n\t\t\t\t\ttitle=\"Colony formation\"\n\t\t\t\t\talt=\"Colony formation\"\n\t\t\t\t\tdata-action=\"\"\n\t\t\t\t\tdata-color-scheme=\"\"\n\t\t\t\t\ttarget=\"\"\n\t\t\t\t\tclass=\"more-info-area\"\n\t\t\t\t\t>\n\t\t\t\t\t<\/map>\n\n\t\t\n\t\t\t\t\t\n\t\t\t\t\t\t<div class=\"hotspot-info \" id=\"hotspot-hotspot-7-0\">\n\t\t\t\t\n\t\t\t\t<h2 class=\"hotspot-title\">Cell culture Transfection passage<\/h2>\t\t\t\t\t\t\t\t<div class=\"hotspot-content\">\n\t\t\t\t\t<p><a href=\"https:\/\/youtu.be\/_d902QFno9s\">Cell culture<\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/zOe0yr6FXEs\">Transfection<\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/ZxeuxcXr0E0\">Transwell<\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/mJOv-EVRrSQ\">Wound healing<\/a><\/p>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t<div class=\"hotspot-info \" id=\"hotspot-hotspot-7-4\">\n\t\t\t\t\n\t\t\t\t<h2 class=\"hotspot-title\">Western blot<\/h2>\t\t\t\t\t\t\t\t<div class=\"hotspot-content\">\n\t\t\t\t\t<p><a href=\"https:\/\/youtu.be\/rn_T1y3BA4A\"><span style=\"color: #0000ee\"><u>Western cell lysate preparation<\/u><\/span><\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/aFW_exhuS2g\">Western gel preparation<\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/Z1q34SZ0Mpg\">Western SDS page<\/a><\/p>\n<div id=\"gtx-trans\">\n<div class=\"gtx-trans-icon\"><\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t\t\t\t\t\t\t\t\t\n\t\t\t\t\t\t<div class=\"hotspot-info \" id=\"hotspot-hotspot-7-7\">\n\t\t\t\t\n\t\t\t\t<h2 class=\"hotspot-title\">Colony formation<\/h2>\t\t\t\t\t\t\t\t<div class=\"hotspot-content\">\n\t\t\t\t\t<p><a href=\"https:\/\/youtu.be\/VtE0O2pwkdA\">Colony formation<\/a><\/p>\n<p><a href=\"https:\/\/youtu.be\/T0sscQ-_mbs\">Staining of colony formation<\/a><\/p>\n<div id=\"gtx-trans\">\n<div class=\"gtx-trans-icon\"><\/div>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t<\/div>\n\t\t\t<\/div>\n\n<\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-29","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/posts\/29","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/comments?post=29"}],"version-history":[{"count":3,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/posts\/29\/revisions"}],"predecessor-version":[{"id":71,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/posts\/29\/revisions\/71"}],"wp:attachment":[{"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/media?parent=29"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/categories?post=29"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/celllab.thu.edu.tw\/index.php\/wp-json\/wp\/v2\/tags?post=29"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}